. 2004 Mar 18;101(13):4519–4524. doi: 10.1073/pnas.0400843101

Table 1. Relative recombination frequencies for various spo11 mutants.

SPO11 genotype	Frequency His+, ×10³ (percent of wild type)^*	rad50S DSBs, percent of total DNA (percent of wild type)^†
SPO11 / SPO11	18.9 ± 3.6 (≡ 100)^‡	9.8 ± 1.8 (≡ 100)
spo11-HA/spo11-HA	11.8 ± 5.8 (62)^‡	2.6 ± 0.40 (27)
spo11-Y135F-HA/spo11-HA	4.4 ± 2.2 (23)^‡	1.7 ± 0.34 (17)
spo11-D290A-HA/spo11-D290A-HA	0.84 ± 0.35 (4.4)	1.2 ± 0.27 (12)
spo11-Y135F/spo11-HA	0.12 ± 0.046 (0.63)^‡	0.42 ± 0.08 (4.3)

Intragenic recombination at his4X::LEU2/his4B::LEU2, measured as the frequency of His + prototrophs per viable cell after 8 h in sporulation medium (mean ± SD of at least three determinations).

^†

DSB frequencies were measured at his4LEU2 site I in rad50S derivatives of the strains used for return-to-growth measurements (mean ± SD of at least two determinations).

^‡

Data are from ref. 37.