Skip to main content
. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: J Dent. 2010 Nov 23;39(2):10.1016/j.jdent.2010.11.005. doi: 10.1016/j.jdent.2010.11.005

Table I.

Chemicals used in the experiment.

Solutions and chemicals Batch number (Manufacturer)
Ortho-Phosphoric acid 0000124351 (Panreac, Barcelona, Spain)
Deionized water 0000201888 (Panreac)
Anhydrous calcium sulphate, Drierite, 6 mesh 14796TJ (Sigma Aldrich, St. Louis, MO)
0.9% Sodium chloride 5211B1 (Braun Medical SA, Barcelona, Spain)
20 % Chlorhedixine digluconate 9418600021 (Guinma, Valencia, Spain)
HEPES buffer, Sterile, pH 8.0 (1 M) 8D007674 (Applichem GmbH, Darmstadt, Germany)
Sodium chloride 2-hydrate powder 0000159010 (Sigma-Aldrich)
Zinc chloride 0000144046 (Sigma-Aldrich)
MMP-2 active, Recombinant CHO cells (0.1 mg/mL) D00065439 (Calbiochem, Gibbstown, NJ)
Borate (di-Sodium tetra-borate anhydrous) 0000173320 (Panreac)
Calcium chloride anhydrous 0000208286 (Panreac)
Glycerol 0000200971 (Panreac)
Brij® 35 0000173320 (Panreac)
Doxycycline C03 (Invicta Farma SA, Madrid, Spain)
Streptomycin sulphate salt 026K8901 (Sigma-Aldrich)
Penicillin G potassium salt 1386789 (Sigma-Aldrich)