Fig. 3.
Expression of Pref-1 and genes involved in respiratory chain function in Tam-treated PPARγad–/– premutant mice. (A) Pref-1 expression was analyzed by RT-PCR performed on RNA extracted from epididymal WAT of CT (lanes 2, 4, 6, and 8) and MT (lanes 3, 5, 7, and 9) mice at D0, D7, D14, and D42. Hypoxanthine phosphoribosyltransferase was used as an internal control. (B) Transcript levels of the indicated genes analyzed by quantitative RT-PCR on RNA isolated from BAT of CT (filled bars) and MT (open bars) mice at D4. Values are expressed as mean ± SEM (relative to CT). n = 5. *, P < 0.05; **, P < 0.005; #, no statistically significant difference.