Fig. 4.

Histological analysis of BAT of Tam-treated PPARγ^ad–/– premutant mice. Histological sections from paraffin-embedded interscapular BAT from CT (aP2-Cre-ER^T2(0/0)/PPARγ^L2/+) mice at D4 (A and F) and MT mice (B–E and G–J) at D4 (B and G), D7 (C and H), D14 (D, I, and J), and D42 (E). (A–I) Hematoxylin and eosin staining. (J) Trichrome staining. (K–O) EM. (K) CT (aP2-Cre-ER^T2(0/0)/PPARγ^L2/+) at D4. (Inset) Typical mitochondria. (L–N) MT at D4. (O) mutant at D14. White arrows point to fused lipid droplets (B and G) and small lipid droplets (O); blue arrows point to necrotic area (C and H) and clumped chromatin (L); green arrows point to detached nuclear membrane (L); red arrows point to lymphocytes (D and I); orange arrows point to fibroblast-like cells (J); purple arrows point to glycogen (O). Lp, lipid droplet; Nc, nucleus; m, mitochondria; hm and sm, hypertrophic and swollen mitochondria, respectively. (Scales are indicated in each image.)