Table 1. Oligonucleotides used in C2CA.
Sequence 5′-3′ | |
---|---|
Padlock probes | |
ppFD+ | P-CCTCCCATCATATTAAAGGCTTTCTCTATGTTAAGTGACCTACGACCTCAATGCTGCTGCTGTACTACTCTTCCTAAGGCATTCTGCAAACAT |
ppMT+ | P-CTGCCATCTTAACAAACCCTCATGATTAGAGCGCATCTGCAATAGCTGCTGTACTACTCTCTTCTTCTATGCGATTACCGGGCC |
ppREF+ | P-CTGCCATCTTAACAAACCCTTACATCTCGGAATCATGCTGGCTGCTGTACTACTCTCTTCTTCTATGCGATTACCGGGCT |
Targets | |
tFD- | GCCTTTAATATGATGGGAGGATGTTTGCAGAATGCCTTAG |
tMT- | GTTTGTTAAGATGGCAGGGCCCGGTAATCG |
tREF- | TTGTTAAGATGGCAGAGCCCGGTAATCGCA |
Molecular beacons | |
mbFD+ | HEX-ccucAAUGCUGCUGCUGUACUACgagg-DABCYL |
mbFD- | HEX-ccucCGUAGUACAGCAGCAGCAUUgagg-DABCYL |
mbREF+ | HEX-cagctACATCTCGGAATCAagcug-DABCYL |
mbMT+ | FAM-gcagCATGATTAGAGCGCATcugc-DABCYL |
Replication oligonucleotides | |
RSA- | GCTGCTGTACTACTCTCTT |
RSA+ | AAGAGAGTAGTACAGCAGC |
FNU- | GACCGTTAGCAGCATGATTCCG |
FNU+ | GAATCATGCTGCTAACGGT |
Sandwich primers | |
WDP-F+ | FITC-CCGAGATGTACCGCTATCGT |
WDP-T+ | TAMRA-AGAGCGCATGAATCCGTAGT |
P, 5′ phosphate; the stem parts of 2′-O-Me-RNA molecular beacons are shown in lowercase.