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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Cell Stem Cell. 2008 Oct 9;3(4):10.1016/j.stem.2008.07.027. doi: 10.1016/j.stem.2008.07.027

Figure 2. Differential gene expression in Stella-positive and Stella-negative population of stella-gfp SH10.10 ESCs.

Figure 2

(A) Q-PCR analysis of each gene in FACS-sorted Stella-GFP-positive and Stella-GFP-negative populations. Relative levels of each gene expression are estimated by referring to the value of Gapdh. The mean values are calculated from three independent experiments. (B) Q-PCR analysis using randomly selected single cells from stella-gfp ESCs. Left graph shows the putative number (log10) of transcript in each single cell. Three populations, Stellahigh, Stellalow and Stellanegative were identified and are set apart by broken lines. Right graph shows the average number of transcripts in each population.