Fig. 4.

Binding, uptake, and outgrowth of mycobacteria by Mϕ-1 and Mϕ-2. (A) Binding (at 0°C) and/or uptake (at 37°C) of an M. bovis bacillus Calmette–Guérin-GFP reporter strain was determined by flow cytometry. After 20 min, more Mϕ-2 than Mϕ-1 had acquired fluorescent mycobacteria (representative of three independent experiments). (B) Accordingly, over a wide range of multiplicities of infection (MOI) from 20 to 2.5 mycobacteria per host cell, Mϕ-2 displayed enhanced outgrowth of M. bovis bacillus Calmette–Guérin-lux at day 6 compared with Mϕ-1. Depicted are averaged luminescence signals as cps [+ standard deviation (n = 4)]. Similar results were found with cells from different donors in four independent experiments.