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. Author manuscript; available in PMC: 2014 Nov 4.
Published in final edited form as: Chem Commun (Camb). 2013 Nov 4;49(85):10.1039/c3cc45752d. doi: 10.1039/c3cc45752d

Fig. 3.

Fig. 3

Spatiotemporally controlled imaging of microtubules via laser-triggered docetaxel-directed photoclick chemistry with terthiophene-tetrazole 6. (a) Reaction scheme. (b) Confocal micrographs of CHO cells treated with 40 μM tetrazole 6 in the presence of (top row) or absence (bottom row) of 30 μM IPFA-docetaxel. The cells in the quadrangle areas were exposed to 405 nm laser irradiation (0.15 mW) at 1.27 μs/pixel illumination dwell followed by scanning entire viewing areas with 458 nm laser at 6.30 μs/pixel illumination dwell; the scanning sequence was intermittently repeated for a duration of 125 sec. Scale bar = 50 μm.