Table 1.

Primer sequences for methylation-specific PCR.

DMR	Chr	Chromosomal location GRCh37	Methylated allele size	Unmethylated allele size	Methylated primer	Unmethylated primer	Universal FAM-labelled primer
H19 promotor	11p15	chr11: 2 019 455–2 019 764	pat 295	mat 305	CGTTTGTTAGTAGAGTGCGTTCGCGAGTCG	GGTTGTTTATTGTTTGTTAGTAGAGTGTGTTTGTG	ATAACAGAAAAAACCCCTTCCTACCACCATCAC
IGF2P0	11p15	chr11: 2 169 485–2 169 651	pat 155	mat 163	GTTTGACGAGGTTAGTGAGGGACGGCG	ATAGTTTTGTTTGAtGAGGTTAGTGAGGGATGGTG	CCAAAACAATTTCCCTAAAAATACTCATTCATAC