. 2013 Nov 18;2(4):225–235. doi: 10.1530/EC-13-0065
This work is licensed under a Table 2.
Primer sequences for pyrosequencing.
| DMR | Chr | Chromosomal location GRCh37 | Methylated allele size | Unmethylated allele size | Primer 1 | Primer 2 (biotinylated) | Sequencing primer(s) |
|---|---|---|---|---|---|---|---|
| IGF2 DMR0 | 11p15 | chr11: 2 169 328–2 169 582 | NA | NA | TGAGGATGGGTTTTTGTTTGGTAT | TCCTCAATCCACCCAAAATAATAT | AAAAGTTATTGGATATATAGT or GGGGTGGAGGGTGTA |
| H19 ICR1 | 11p15 | chr11: 1 977 650–1 977 877 | NA | NA | GTATAGTATATGGGTATTTTTGGAGG | CCATAAATATCCTATTCCCAAATAACC | GTTTYGGGTTATTTAAGTT |