Table 6.

Additional validation of gene expression data. Expression of genes identified as being up- or downregulated in the microarray were assessed with Q-PCR. Relative fold expression for each of the seven genes analysed is given for each of the four 3-M cell lines. Expression was normalised to GAPDH and mean control cell line expression.

Gene	CUL7		OBSL1 F		OBSL1 M		CCDC8
Relative expression	P	Relative expression	P	Relative expression	P	Relative expression	P
BEX1	0.06±0.01	<0.001	0.15±0.20	<0.001	0.03±0.02	<0.001	0.20±0.24	<0.001
LEP	0.07±0.04	<0.001	0.06±0.03	<0.001	0.01±0.00	<0.001	0.04±0.01	<0.001
ZIC1	946±462	<0.001	496±186	0.004	1200±891	<0.001	759±498	0.002
HOXC6	31±24	0.006	88±55	0.001	51±38	0.005	100±87	0.009
HOXA9	690±586	0.008	501±466	0.012	349±296	0.008	399±342	0.008
GPC6	35±6	<0.001	44±10	<0.001	7±2	<0.001	5±1	<0.001