Table 2.
List of plasmids and strains in this work
| Plasmids | Characteristic(s) | Reference |
|---|---|---|
| pET28a(+) |
KanR; T7 promoter based E. coli expression vector |
Novagen |
| pET28a-ACP |
pET28a(+) derivative carrying M. purpureus ACP encoded DNA |
This study |
| pET28a-ACPm |
pET28a(+) derivative carrying M. purpureus ACPm encoded DNA |
This study |
| pESC-ATX |
AmpR; URA3; PGAL1/GAL10-based yeast expression vector; commerial pESC-URA derivative carrying atX gene |
Moriguchi et al., 2006 |
| pPIC3.5 K |
AmpR G418R; HIS4; PAOX1-based yeast expression vector |
Invitrogen |
| pPICZ B |
ZeocinR ; PAOX1-based expression yeast vector |
Invitrogen |
| pPIC3.5 K-NpgA |
pPIC3.5 K derivative carrying A. nidulans npgA gene |
This study |
| pPICZ B-NpgA-HIS6 |
pPICZ B derivative carrying A. nidulans npgA gene |
This study |
| pPICZ B-ATX |
pPICZ B derivative carrying A. terreus atX gene |
This study |
| pPICZ B-CT-SKL |
pPICZ B derivative carrying M. purpureus pksCT gene a P. pastoris SKL sequence |
This study |
| pPICZ B-CT |
pPICZ B derivative carrying M. purpureus pksCT gene |
This study |
| pPIC3.5 K-NpgA-SKL |
pPIC3.5 K derivative carrying A. nidulans npgA gene and a P. pastoris SKL sequence |
This study |
|
Strains |
Characteristic(s) |
Reference |
| GS115 |
his4, AOX 1, AOX2 |
Invitrogen |
| GS115-NpgA-HIS6 |
ZeocinR; GS115 with plasmid pPICZ B-NpgA-HIS6 |
This study |
| GS115-ATX |
ZeocinR; GS115 with plasmid pPICZ B-ATX |
This study |
| GS115-NpgA |
G418R; GS115 with plasmid pPIC3.5 K-NpgA |
This study |
| GS115-NpgA-ATX |
ZeocinR; G418R; GS115-NpgA with plasmid pPICZ B-ATX |
This study |
| GS115-3.5 K |
G418R; GS115 with pPIC3.5 K |
This study |
| GS115-3.5 K-CT-SKL |
ZeocinR; G418R; GS115-3.5 K with pPICZ B-CT-SKL |
This study |
| GS115-NpgA-CT |
ZeocinR; G418R; GS115-NpgA with plasmid pPICZ B-CT |
This study |
| GS115-NpgA-SKL |
G418R; GS115 with plasmid pPIC3.5 K-NpgA-SKL |
This study |
| GS115-NpgA-CT-SKL |
ZeocinR; G418R; GS115-NpgA with plasmid pPICZ B-CT-SKL |
This study |
| GS115-NpgA-SKL-CT-SKL |
ZeocinR; G418R; GS115-NpgA-SKL with plasmid pPICZ B-CT-SKL |
This study |
|
E. coli TOP10 |
F’ [lacIq, Tn10(TetR)] mcrA φ80lacZΔM15 ΔlacX74 deoR recA1 |
Invitrogen |
|
E. coli BL21 |
F–ompT hsdSB(rB– mB–) gal dcm (DE3) |
Invitrogen |
| BL21-ACP |
KanR; BL21 with plasmid pET28a-ACP |
This study |
| BL21-ACPm | KanR; BL21 with plasmid pET28a-ACPm | This study |