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. 2004 Mar 16;101(13):4649–4654. doi: 10.1073/pnas.0400590101

Fig. 1.

Fig. 1.

Immunohistochemical analysis of brains from transgenic mice. Anti-orexin-A immunostaining of coronal sections of brain tissue from wild-type, orexin/ataxin-3-hemizygous-transgenic-, CAG/orexin-hemizygous-transgenic-, and double-hemizygous-transgenic mice were performed as described (31). Note the punctate staining of native orexin-A-immunoreactive neurons clustered in the perifornical LH of wild-type mice (a and b) and the absence of orexin-A in the brains of orexin/ataxin-3-transgenic mice in which native orexinergic neurons have degenerated (c and d). CAG/orexin-transgenic mice have widespread, diffuse, ectopic production of orexin-A in addition to that observed in native neurons (e and f). In contrast, orexin/ataxin-3;CAG/orexin-double-transgenic mice exhibit only the ectopic pattern of orexin-A; native neurons are absent (g and h). (a Inset) The location and size of magnified images in b, d, f, and h. f, fornix; Arc, arcuate nucleus of the hypothalamus; 3V, third ventricle. (Bar, 3 mm for a, c, e, and g and 0.5 mm for b, d, f, and h).