Fig. 1.
Neural deletion of STAT3 results in severe obesity. (a) Western blot analysis of STAT3 in forebrain, hypothalamus, and cerebellum in WT and KO animals. The blot was stripped and reprobed with a STAT1 Ab as a control. (b) Heterozygous and obese homozygous Nestin-Cre; STAT3f/f (STAT3N-/-) animals compared to a db/db mutant. (c) NMR imaging by using a minispec MQ10 analyzer (Bruker, Woodlands, TX) of WT, KO, and db/db animals (n = 4) showing total fat and lean mass as well as free fluid. Data are given as percentage of total body weight.