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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Scand J Immunol. 2013 Nov;78(5):10.1111/sji.12106. doi: 10.1111/sji.12106

Figure 1. 4T1-12B mouse mammary carcinoma cells secrete TSLP.

Figure 1

4T1-12B cells were grown in culture and cytokine concentrations were measured in the supernatant (A). Primary tumors from WT and Tslpr−/− mice were dissociated and the resulting cells were counted (B). Equal numbers of cells were plated for each group and cytokines levels were measured in the supernatants. Figure 1A shows that the mouse breast cancer cell line 4T1-12B secretes large amounts TSLP protein in culture. Figure 1B shows that TSLP is also produced by the primary tumor in both WT and Tslpr−/− mice bearing primary tumors (B). Concentrations of cytokines were compared using a Student’s t test (n=3).