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. Author manuscript; available in PMC: 2014 Sep 27.
Published in final edited form as: Circ Res. 2013 Aug 1;113(8):10.1161/CIRCRESAHA.113.301934. doi: 10.1161/CIRCRESAHA.113.301934

Figure 7. IL-17A ablation abolishes enhanced aortic macrophage accumulation in renal impairment.

Figure 7

The aortic leukocyte infiltrate was analyzed in LDLr−/− mice reconstituted with either wt or Il17a−/− bone marrow and normal and impaired renal function. (A) Immunofluorescence to assess CD11b (green) and CD11c (red) in the aortic root (typical examples of aortic valves, rectangles mark the cell rich intimal regions shown in zoom. 20x orig. magn., bars=100μm). (B) Number of total aortic CD11b+ and CD11c+ cells determined by flow cytometry (n=5-8, 4 independent experiments). (C,D) Aortic cell proliferation was assessed by Ki67 staining (E, examples of aortic valve area plaques, 10x orig. magn., bar=200μm, F: mean Ki67+ cell numbers/aortic section from n=4 sections/mouse, n=4-6 mice/group, One-way-ANOVA and Bonferroni post-hoc test).