Table 1.
Kinetic parameters of MGL from various sources*
| Substrate | MGL from P. gingivalis | MGL from C. tetani | MGL from C. sporogenes | MGL from C. freundii** | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
kcat, s-1 |
Кm, mM |
kcat /Km, M-1s-1 | kcat, s-1 |
Кm, mM |
kcat /Km, M-1s-1 |
kcat, s-1 |
Кm, mM |
kcat /Km, M-1s-1 |
kcat, s-1 |
Кm, mM |
kcat /Km, M-1s-1 | |
| L-Met | 3.9 | 1.77 | 2.2×103 | 12 | 0.947 | 1.27×104 | 9.86 | 0.432 | 2.28×104 | 6.2 | 0.7 | 8.85×103 |
| S-Et-L-Hcy | 3.84 | 0.93 | 4.13×103 | 5.89 | 0.545 | 1.08×104 | 7.05 | 0.278 | 2.54×104 | 6.78 | 0.54 | 1.26×104 |
| L-Met(SO) | 5.05 | 12.22 | 4.13×102 | 2.7 | 7.07 | 3.82×102 | 6.7 | 33.51 | 2.0×102 | 2.52 | 6.21 | 4.06×102 |
| S-Et-L-Cys | 8.05 | 2.17 | 3.71×103 | 7.08 | 0.72 | 9.83×103 | 6.3 | 0.358 | 1.76×104 | 5.03 | 0.17 | 2.96×104 |
| S-Bzl-L-Cys | 5.8 | 1.47 | 3.94×103 | 8.5 | 0.766 | 1.11×104 | 10 | 0.348 | 2.87×104 | 8.16 | 0.18 | 4.53×104 |
* The mean squared error of the experiment in the determination of the kinetic parameters did not exceed 10%.
** Data from [16, 17].