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. 2004 Mar 19;101(13):4690–4694. doi: 10.1073/pnas.0306924101

Fig. 1.

Fig. 1.

Generation of wild-type and mutant WNK4-expressing MDCK II cell lines. (a) Expression of wild-type and mutant WNK4 in MDCK II cells. HA-tagged WNK4 proteins were detected by using an anti-HA (9Y10) Ab. Expression of WNK4 was induced by removal of doxycycline from the medium for 4 days. W28 and W31 are wild-type WNK4-expressing cell lines, and M18 and M19 are mutant WNK4-expressing cell lines. (b) Immunofluorescence of wild-type and mutant WNK4 expressed in isolated stable cell lines. Cells were grown on a permeable support, and the expression of WNK4 was induced for 4 days. WNK4 was detected by using an anti-HA Ab in conjunction with an Alexa 546-conjugated anti-rat IgG Ab. Cells were stained for occludin by using an anti-occludin Ab (Zymed) in conjunction with an Alexa 488-conjugated anti-rabbit IgG Ab.