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. 2012 Apr 23;23(2):137–147. doi: 10.1089/hgtb.2012.054

FIG. 3.

FIG. 3.

Specificity of in vitro transduction. (A) IL-13Rα2-expressing HOS cells were transduced with EGFP-encoding lentiviral vectors pseudotyped either with HcΔ18-AA-IL-13/FcΔ30 glycoprotein (right) or FcΔ30/HcΔ18-AA glycoprotein (left). (B) IL-13Rα2-expressing HOS cells were treated with a goat anti-IL-13Rα2 polyclonal antibody before and during transduction; MV, MV HcΔ18-AA-IL-13/FcΔ30-pseudotyped lentiviral vector; VSV-G, VSV-G-pseudotyped lentiviral vector. (C) The pseudotyped lentiviral vectors were treated with recombinant rIL-13Rα2, the soluble extracellular domain of IL-13Rα2, before and during transduction; MV, MV HcΔ18-AA-IL-13/FcΔ30-pseudotyped lentiviral vector; VSV-G, VSV-G-pseudotyped lentiviral vector.