Effects of leptin and PYY3-36 on feeding in Pomc-/- and wild-type mice. (A and B) Response of 12- to 15-week-old (A; n = 6 for all groups) and 4-week-old (B; n = 4-7) freely feeding, male wild-type and Pomc-/- mice to peripherally administered leptin. Data represent mean ± SEM daily food intake over 4 days of saline (s) or leptin (L) treatment. Leptin vs. saline, *, P < 0.05, **, P < 0.01. (C) Acute feeding response of 12- to 15-week-old, fasted, male wild-type and Pomc-/- mice to PYY3-36 or saline administered i.p. Data represent mean ± SEM food intake 4 h after saline or PYY3-36 treatment. n = 8 for all groups. PYY3-36 (P) vs. saline (s), **, P < 0.01.