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. 2013 Dec 15;140(24):4926–4936. doi: 10.1242/dev.099879

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© 2013. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Regulation of prespore/spore and prestalk/stalk differentiation by ChdC. (A) RNA was prepared from 12-hour developed cells, and relative expression levels of the prespore genes pspA and cotB and the prestalk genes ecmA and ecmB were quantified by RT-qPCR. (B) Wild-type and chdC-null cells were transformed with either the prespore-specific expression construct pspA/lacZ or the prestalk-specific expression construct ecmA/lacZ, plated for development on nitrocellulose filters and stained for β-galactosidase activity.