SEB-3 mediates heat stress-induced and ethanol-withdrawal-induced tremor. A, Heat evoked tremor in N2, but not in seb-3 (lf) animals (*P < 0.0001 by Fisher’s exact test). seb-3 (eg696 gf) displayed tremor without heat stress. [* P < 0.0001 by Fisher’s exact test; n=75 (N2, 22°C), 95 (N2, 37°C), 94 (seb-3 (eg696 gf), 22°C), 80 (seb-3 (eg696 gf), 37°C), 85 (seb-3 (tm1848 lf), 22°C), and 97 (seb-3 (tm1848 lf), 37°C)]. B, Acute ethanol withdrawal (300mM) induced a tremor in N2 animals (n=50) and the incidence of tremor was greater after 3 days (n=54) of ethanol exposure than after 4 h (n=55) of exposure (* P<0.001, ** P <0.0001 by Fisher’s exact test). Reapplication of ethanol completely abolished tremor (n=54). C, Ethanol withdrawal (400mM) induced a tremor in seb-3 mutants but seb-3 (lf) animals (n=91) showed much less tremor than wild type, N2 animals (n=60) (* P < 0.0001 by Fisher’s exact test).