FIG. 1.

Fluorescence-based screening of one-bead–one-compound (OBOC) library. A linear pentameric library was synthesized on beads using a split-mix method for screening as described in Materials and Methods. Only a small portion of the entire image is presented in A. The positive human serum albumin (HuSA)-phycoerythrin (PE) binding beads are red and C-reactive protein–fluorescein isothiocyanate (CRP-FITC)-binding beads are green. Both HuSA- and CRP-binding beads are orange (B). Some beads have no color, meaning there was no binding to CRP and/or HSA. The green positive beads were resynthesized on bead for reconfirmation. The CRP strong binding CRPi-2 (C) and weakly binding CRPi-6 (D) are shown here. Autofluorescent beads were removed from the library.