Figure 4.

TSG inhibited LPS-induced ROS production and NADPH oxidase activation. The extracellular superoxide level was measured by SOD-inhibitable reduction of WST-1 (a), and the content of intracellular ROS were detected with DCFH-DA (b). The subcellular fractions were isolated, and western blot assay was performed to evaluate the NADPH oxidase subunit p47 levels in the cytosolic and membrane fractions of BV2 cells. β-actin and gp91 were applied as the internal cytosolic and membrane controls, respectively, (c). The ratio of densitometry values of cytosolic and membrane p47 compared with β-actin and gp91, respectively, was assessed and normalized to each control cultures (d). Results were expressed as a percentage of the control cultures and were the mean ± SEM from three independent experiments performed in triplicate. ^# P < 0.05 compared with control cultures; *P < 0.05 compared with LPS-treated cultures.