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. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Anal Biochem. 2013 Aug 6;442(2):10.1016/j.ab.2013.07.039. doi: 10.1016/j.ab.2013.07.039

Figure 4. SNV induces increase in GTP-bound Rho GTPase levels in Vero E6 cells.

Figure 4

Bar graph shows GLISAs measuring kinetics of adhesion-dependent Rho protein (Rac1 and Cdc42) activation. PI-PLC-treated cells were cleared of DAF surface expression, a receptor for virus binding. Serum starved cells were stimulated with SNV particles as described in the methods and compared with unstimulated cells (0 min). Control cells were mock-treated with media to account for virus diluent. After cell lysis, the amounts of active Rac1 and RhoA were quantified based on p21-activated protein kinase or Rhotekin binding by GLISA respectively. The error bars represent the standard deviation of three independent measurements.