Skip to main content
. Author manuscript; available in PMC: 2014 Nov 15.
Published in final edited form as: Anal Biochem. 2013 Aug 6;442(2):10.1016/j.ab.2013.07.039. doi: 10.1016/j.ab.2013.07.039

Figure 5.

Figure 5

SNV induces the activity of several GTPases in Vero E6 cells. A. Vero E6 cells were serum starved for 24 h and treated with 10,000 SNVR18/cell. Active Rac1 and RhoA were detected in cell lysates at 3 min and 20 min after virus exposure using PAK1 and Rhotekin beads, respectively.. The errors represent standard deviations in 3 separate measurements. C. Rap1 and H-Ras were sequentially activated by SNVR18 in Vero E6 cells. Vero E6 cells were serum starved for 24 h and treated with 10,000 SNVR18/cell. Active Rap1 and H-Ras were measured on Ral and Raf-functionalized beads, respectively, at 3 and 20 min after virus exposure. Cells were treated with the cAMP analog 8-pCPT-2′-O-methyl-cAMP (O-Me-cAMP), which stimulates the Epac/Rap1 pathway as a specificity control. Error bars represent standard deviations 3 separate measurements. D. Rab7 is activated in response to SNV exposure. Cell lysates prepared at varying timepoints (0–60 min) following SNV exposure were analyzed for the presence of active Rab7 using RILP functionalized beads. Error bars represent 3 separate measurements. ** P<0.001 for all data compared to resting (rest) cells.