Figure 3.

CO attenuates expression of GSK-3β signaling in human macrophage cell lines and MLNs. (a) U937cells were incubated with CORM2 (100 μM) for various time periods (0, 10, 20, 30, and 60 min) (b) Cells were treated with CORM2 in a dose dependent manner (0, 10, 20, 30, 50, and 100μM) for 30 min. (c) U937cells were preincubated with CORM2 (100 μM) and LiCl (20 mM) for 30 min and then stimulated with LPS (1 μg/mL) for 30 min. (d) MLNcells were preincubated with CORM2 (100 μM) and LiCl (20 mM) for 30 min and then stimulated with LPS (1 μg/mL) for 30 min. Protein expressions of pGSK-3β and pIκB were detected by western blotting.