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Thermal unfolding curves of wild type or mutant chitosanases in the absence or presence of chitosan. (A) wild type. (B) R42K chitosanase. (C) R42E chitosanase. (a) no chitosan. (b) 82.5% DDA chitosan. (c) 99% DDA chitosan. The unfolding process was monitored by intrinsic tryptophan fluorescence.
