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Transition temperatures of thermal unfolding of wild type and R42-mutated CsnN174 chitosanases in the absence or presence of two chitosans with different degrees ofN-deacetylation
| |
|
Tm(°C) |
|||
|---|---|---|---|---|---|
| Enzyme | Enzyme only | Enzyme + chitosan (DDA 84%) | Enzyme + chitosan (DDA 99%) | Chitosan (DDA 84%) | Chitosan (DDA 99%) |
| WT |
48.8 |
53.4 |
53.2 |
4.6 |
4.4 |
| R42E |
43.2 |
59.5 |
62.5 |
16.3 |
19.3 |
| R42K | 47.7 | 62.5 | 66.1 | 14.8 | 18.4 |
