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Relative cleavage frequencies of wild type and mutated CsnN174 chitosanases from (GlcN)6 hydrolysis at 50% of substrate depletion calculated from data on Figure 7
| |
Symmetrical cleavage |
Asymmetrical cleavage |
||
|---|---|---|---|---|
| Enzyme | 6 → 3 + 3 | 4 → 2 + 2 | Total | 6 →4 + 2 |
| WT |
5,14 (60%) |
0,28 (3%) |
5,42 (63%) |
3,19 (37%) |
| R42E |
5,66 (66%) |
0,65 (8%) |
6,21 (74%) |
2,23 (26%) |
| R42K | 5,73 (65%) | 0,53 (6%) | 6,26 (71%) | 2,60 (29%) |
