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. 2013 Sep 14;14:622. doi: 10.1186/1471-2164-14-622

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Copyright © 2013 Arenas et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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In vitro recombination in the TPS islands. (A) Organization of the TPS islands in strain B16B6 and its mutant derivative B16B6ΔtpsC2-5 where a kan cassette replaces several tpsC cassettes and IORFs. (B) Organization of the TPS island in recombinant strain α14-tpsA* (middle) obtained after transformation of strain α14 (top) with chromosomal DNA from strain B16B6ΔtpsC2-5 (bottom). (C) Organization of the TPS island in recombinant strain α153-tpsA* (middle) obtained after transformation of strain α153 (top) with chromosomal DNA from strain α14-tpsA*#1 (bottom). The organization of the TPS island of strain α153 is not completely depicted because it is not located on a single contig in the available genome sequence.