Table 1.
Groups | Cleavage/oocytes (%) | Blastocyst/cleavage (%) |
---|---|---|
1. CTR, untreated | 68/116 (59%) | 22/68 (32%) |
2. Hoechst | 28/45 (62%) | 10/28 (36%) |
3. UV (both cytoplasm and MII plate) | 9/119 (8%)a | 0/9 (0%)a |
4. UV (only cytoplasm) | 20/95 (21%)b | 0/20 (0%)b |
5. Cytochalasin B | 48/90 (53%) | 6/48 (13%)c |
6. Demecolcine | 37/60 (62%) | 5/37 (13%)d |
Treated with:
UV (cytoplasm+MII) vs. CTR: cleavage p<0.0001.
UV (only cytoplasm) vs. CTR: cleavage p<00001; blastocysts p=0.0394; blastocysts p=0.0023.
Cytochalasin B vs. CTR: blastocysts p=0.0158.
Demecolcine vs. CTR: blastocysts p=0.0382. Fisher exact test.
CTR, control; UV, ultraviolet; MII, metaphase II.