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. 2013 Sep 14;10:288. doi: 10.1186/1743-422X-10-288

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Copyright © 2013 Qiao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Specificity of RT-LAMP. A, Agarose gel electrophoresis showing the specificity of RT-LAMP on amplification of different viruses. B, Visual detection of RT-LAMP amplification products of the different viruses using SYBR Green I staining. The color change from orange in the negative to green in the positive reaction. The lane and tube numbers correspond to the following specimens: Lanes 1, 2, 4 and 5, Akabane virus strain JaGAR 39/MB19; Lane 3, Bovine herpesvirus 1 strain 758; Lane 6, Bovine parvovirus strain BF15; Lane 7, Bluetongue virus strain CSIRO 154; Lane 8, Bovine viral diarrhea virus strain NADL; Lane M, 100-bp DNA Ladder.