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Sensitivity of RT-LAMP. The sensitivity of RT-LAMP was determined using 10-fold serial dilutions of the culture supernatant contain 5×105 TCID50/mL of Akabane virus. The lane numbers correspond to the following specimens: Lane 1, 10 –1 dilutions; Lane 2, 10 –2 dilutions; Lane 3, 10 –3 dilutions; Lane 4, 10 –4 dilutions; Lane 5, 10 –5 dilutions; Lane 6, 10 –6 dilutions; Lane 7, 10 –7 dilutions; Lane 8, 10 –8 dilutions; Lane M, 100-bp DNA Ladder.
