Table 2.
Oligonucleotide primers used for RT-LAMP assay and semi-nested RT-PCR against Akabane virus
| Primers | Sequence (5'-3') | Nucleotide positions* | Length (bp) |
|---|---|---|---|
| F3 |
TGTTCCACAACGGAATGC |
27–44 |
18 |
| B3 |
TGTAAATTTGACCCCTGCA |
198–216 |
19 |
| FIP |
GAGCTGCTGCCCATACTTACT-AGCTACATTTAATCCGGATGC |
|
42 |
| BIP |
TACTGTTGCTAGAGTCTTCTTCCTC-AGTAAGATCGACACTTGGTT |
|
45 |
| N1 |
ATGTTCCACAACGGAATGCAGC |
25–46 |
22 |
| N2 |
AGCCAGGAAAGCTCTAGCTGCAG |
680–658 |
23 |
| N3 | GGAAAATGGTTATTAACCACT | 215–235 | 21 |
*Nucleotide sequence position according to nucleoprotein (N) gene of Akabane virus strain 93FMX (GenBank accession number FJ498797).