Table 1.
Primers and probes used for real-time PCR
| Gene | Primer 1 | Primer 2 | Probe |
|---|---|---|---|
| GAPDH |
TGTAGTTGAGGTCAATGAAGGG |
ACATCGCTCAGACACCATG |
AAGGTCGGAGTCAACGGATTTGGTC |
| MFN2 |
ATGCATCCCACTTAAGCAC |
CCAGAGGGCAGAACTTTCTC |
AGAGGCATCAGTGAGGTGCT |
| PGC-1α |
ATAAATCACACGGCGCTCTT |
TGAGAGGGCCAAGCAAAG |
AGAGGCAGAGGCAGAAGG |
| UCP3 |
CAAAATCCGGGTAGTGAGGCT |
TGACTCCGTCAAGCAGGTGTAC |
CCCCCAAAGGCGCGGACAAC |
| GLUT4 | TCTTCACCTTGGTCTCGGTGTTGT | CACGAAGCCAAAGATGGCCACAAT | ATGTGTGGCTGTGCCATCCTGATGA |
GAPDH Glyceraldehyde 3-phosphate dehydrogenase, MFN2 mitofusin 2, PGC-1α peroxisome-proliferator- activated receptor-gamma co-activator 1 alpha, UCP3 uncoupling protein 3, GLUT4 glucose transporter 4.