FIG. 2.

Characterization of PBMC-derived iPSCs. (A) RT-PCR analysis for hESC marker genes OCT4, SOX2, NANOG, and HPRT as a loading control. Samples were: (1) PBMCs after SeV vector transduction; (2) hiPSCs passage2; (3) hiPSCs passage 7; (4) hiPSC passage 13; (5) hESC H9. Expression analysis (left panel) of 90 well-defined gene markers for pluripotency indicate similarity between PBMC-derived iPSCs and H9 hESCs. (B) Immunofluorescence staining for hESC markers OCT4, SOX2, NANOG, TRA-1-60, and SSEA-4 (right panel). Bright-field images of PBMC-derived iPSCs (left panel). (C) EBs from PBMC-derived hiPSCs growing in suspension cultures after 15 days. (D) Gross anatomy of different tissues derived from a teratoma of PBMC-derived hiPSCs (H&E). (E) Normal karyotype in both control PBMC-hiPSCs and CMD PBMC-iPSCs. Color images available online at www.liebertpub.com/cell