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T cell receptor gene rearrangement assay. (A) PBMC-derived iPSC lines show no TCR rearrangement in TCRG loci with McCarthy primers. Genomic DNA from: (1) Fibroblasts; (2) peripheral blood; (3–6) iPSCs; (7) no DNA. (B) TCRG examined by BIOMED-2 primer sets and capillary electrophoresis. Specific peaks shown by arrows are seen in positive control (clonal DNA) but not control iPSCs and CMD iPSCs. The two peripheral peaks in each plot are DNA ladders.
