Table 1.

Targetron insertion sites and scores

Gene insertion site	-30 to -1 NT upstream	LtrB intron	+1 to +15 NT downstream	Score
IS605 435|436 s	CCGATTATAGATCATCAAAAATATAGTGTA	gtgc…tcac	GAAATTATTCGCAAA	9.3
BAS4553 343|344 s	GTTCAAATGGATTATACGGATGCACGTACG	gtgc…tcac	GCTTTTTGGAAGTGT	8.4
BAS4597 1794|1795 s	AATCAATTAGATTGGGATCGAAAAGAGAAA	gtgc…tcac	GAAATAGAGACCGTT	7.3

The gene chosen for intron insertion is shown in the first column with the nucleotide positions at which the intron is designed to insert at within the gene shown beside the gene name. All intron insertions are in the sense orientation relative to gene transcription and designated here with the suffix “s”. The sequence of 30 nucleotides upstream and 15 nucleotides downstream of the intron insertion site are shown together with a numeric score reflecting expected efficiency based on a learning set of Ll.Ltrb intron insertions.