Table 2.
Primers used for retargeting
| Primer name | Primer sequence | Pairings changed |
|---|---|---|
| IS605-435|436 s |
AAAAAAGCTTATAATTATCCTTAAAATACAGTGTAGTGCGCCCAGATAGGGTG |
IBS1/2 |
| IS605-435|436 s |
CAGATTGTACAAATGTGGTGATAACAGATAAGTCAGTGTAGATAACTTACCTTTCTTTGT |
EBS1/δ |
| IS605-435|436 s |
TGAACGCAAGTTTCTAATTTCGGTTTATTTCCGATAGAGGAAAGTGTCT |
EBS2 |
| BAS4553-343|344 s |
AAAAAAGCTTATAATTATCCTTAGATGCCCGTACGGTGCGCCCAGATAGGGTG |
IBS1/2 |
| BAS4553-343|344 s |
CAGATTGTACAAATGTGGTGATAACAGATAAGTCCGTACGGCTAACTTACCTTTCTTTGT |
EBS1/δ |
| BAS4553-343|344 s |
TGAACGCAAGTTTCTAATTTCGATTGCATCTCGATAGAGGAAAGTGTCT |
EBS2 |
| BAS4597-1794|1795 s |
AAAAAAGCTTATAATTATCCTTACGAAACGAGAAAGTGCGCCCAGATAGGGTG |
IBS1/2 |
| BAS4597-1794|1795 s |
CAGATTGTACAAATGTGGTGATAACAGATAAGTCGAGAAAGATAACTTACCTTTCTTTGT |
EBS1/δ |
| BAS4597-1794|1795 s |
TGAACGCAAGTTTCTAATTTCGGTTTTTCGTCGATAGAGGAAAGTGTCT |
EBS2 |
| EBS-Universal | AACCGAAATTAGAAACTTGCGTTCA | None |
The primers shown were used in PCR to generate a HindIII BsrG1 fragment that was exchanged into the Targetron vectors and retarget the intron to the genomic location indicated in the primer name. The exact nucleotide the intron is designed to insert at is designated by a | at the genomic location indicated above the primer sequences.