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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Int J Obes (Lond). 2011 Oct 25;36(8):10.1038/ijo.2011.201. doi: 10.1038/ijo.2011.201

FIGURE 1.

FIGURE 1

Representative oxygen consumption tracings from permeabilized primary human myotubes using high resolution respirometry in non-adhered (Panel A; Oroboros Oxygraph 2K) and adhered (Panel B; Seahorse Flux Analyzer XF-24) myotubes. Data for both methodologies are normalized to cell count. Arrows indicate a lack of Cytochrome C response confirming intact mitochondria and circles identify comparable data points for each method. Abbreviations are as presented in Table 2. The relative change over State 4 respiration for the comparable data points is presented in Panel C where the dotted line represents State 4 respiration.