. Author manuscript; available in PMC: 2013 Dec 3.

Published in final edited form as: Int J Obes (Lond). 2011 Oct 25;36(8):10.1038/ijo.2011.201. doi: 10.1038/ijo.2011.201

Table 2.

Respirometry Protocol

Step	Substrate	Notation	Concentration
1	Digitonin	Baseline	7-10 μg/10⁶ cells
2	Palmitoyl Carnitine	PC	5 μmol/l
2	Malate	M	1 mmol/l
3	ADP	D	2 mmol/l
4	Cytochrome C	CytC	10 μmol/l
5	Glutamate	G	2 mmol/l
6	Succinate	S	3 mmol/l
7	Oligomycin	State 2	2.5 μg/ml
8	FCCP	Uncoupled	2 μmol/l

PC, M: PCM₄
PC, M, D: PCM₃
PC, M, G, D: PCMG₃
PC, M, G, S, D: PCMGS₃
S, M, D: SM₃