. Author manuscript; available in PMC: 2013 Dec 3.

Published in final edited form as: Chem Res Toxicol. 2012 Jun 15;25(7):10.1021/tx3002109. doi: 10.1021/tx3002109

Table 1.

Frequency and Site of Mutations in the rpoB Gene in E. coli Cells Produced by BD (20%, in air, v/v) and Its Metabolites (0.3 mM)

base change	codon change	amino acid	mutation	number of mutations
base change	codon change	amino acid	mutation	spontaneous (n = 32)	DEB (n = 43)	DEB + GST (+ GSH) (n =45)	DEB-GSH conjugate (n = 48)	EB (n = 44)	BD (n = 40)	BD + microsomes (n = 42)	BD + microsomes + GST (+ GSH) (n = 41)
G:C to T:A	GAC	516	D → Y	0	6	4	3	6	0	5	3
	TAC
	CAG	513	Q → K	0	3	2	2	0	0	1	2
	AAG
	CGT	529	R → S	0	4	8	8	2	0	4	6
	AGT
	TCC	531	S → Y	0	1	3	2	3	0	3	1
	TAC
	CGT	529	R → L	0	0	3	4	0	2	0	2
	CTT
	CAC	526	H → N	0	0	0	0	4	6	1	1
	AAC
	TCT	512	S → Y	0	0	0	0	0	6	0	0
	TAT
G:C to A:T	CAC	526	H → Y	4	7	2	2	3	3	6	2
	TAC
	CCT	564	P → L	0	4	2	1	5	4	2	1
	CTT
	CGT	529	R → C	0	1	0	0	9	0	3	2
	TGT
	TCC	531	S → F	0	5	2	2	4	7	5	1
	TTC
	GAC	516	D → N	0	0	1	2	0	0	0	2
	AAC
A:T to T:A	CAG	513	G → L	2	5	3	1	2	6	5	2
	CTG
	GAC	516	D → V	0	2	1	1	4	2	4	2
	GTC
	ATC	572	I → F	0	4	1	2	0	4	3	1
	TTC
A:T to C:G	ATC	572	I → L	12	0	0	0	0	0	0	0
	CTC
	CAG	513	Q → P	0	1	6	8	2	0	0	7
	CCG
A:T to G:C	GAC	516	D → G	14	0	0	0	0	0	0	0
	GGC
	CAC	526	H → R	0	0	4	6	0	0	0	4
	CGC
	CAG	513	Q → R	0	0	3	4	0	0	0	2
	CGG