Table 3.

Comparison of Mutation Spectra in the rpoB Gene in E. coli Cells Produced by BD (20%, in air, v/v) and Its Metabolites (0.3 mM)

base change		P-value
		spontaneous	DEB	DEB + GST (+ GSH)	DEB-GSH conjugate	EB	BD	BD + microsomes	BD + microsomes + GST (+ GSH)
G:C to T:A	spontaneous	-	0.0002	0.0001	0.0001	0.0001	0.0001	0.0001	0.0001
	DEB	0.0002	-	NSa	NS	NS	NS	NS	NS
	DEB + GST (+ GSH)	0.0001	NS	-	NS	NS	NS	NS	NS
	DEB-GSH	0.0001	NS	NS	-	NS	NS	NS	NS
	EB	0.0001	NS	NS	NS	-	NS	NS	NS
	BD	0.0001	NS	NS	NS	NS	-	NS	NS
	BD + microsomes	0.0001	NS	NS	NS	NS	NS	-	NS
	BD + microsomes + GST (+ GSH)	0.0001	NS	NS	NS	NS	NS	NS	-
G:C to A:T	spontaneous	-	0.01	NS	NS	0.001	0.03	0.018	NS
	DEB	0.01	-	0.03	0.009	NS	NS	NS	NS
	DEB + GST (+ GSH)	NS	0.03	-	NS	0.003	NS	NS	NS
	DEB-GSH	NS	0.009	NS	-	0.0007	0.04	0.015	NS
	EB	0.001	NS	0.003	0.0007	-	NS	NS	0.011
	BD	0.03	NS	NS	0.04	NS	-	NS	NS
	BD + microsomes	0.018	NS	NS	0.015	NS	NS	-	NS
	BD + microsomes + GST (+ GSH)	NS	NS	NS	NS	0.011	NS	NS	-
A:T to T:A	spontaneous	-	0.034	NS	NS	NS	0.015	0.018	NS
	DEB	0.034	-	0.048	0.045	NS	NS	NS	NS
	DEB + GST (+ GSH)	NS	0.048	-	NS	NS	0.024	0.026	NS
	DEB-GSH	NS	0.045	NS	-	NS	0.012	0.015	NS
	EB	NS	NS	NS	NS	-	NS	NS	NS
	BD	0.015	NS	0.024	0.012	NS	-	NS	NS
	BD + microsomes	0.018	NS	0.026	0.015	NS	NS	-	NS
	BD + microsomes + GST (+ GSH)	NS	NS	NS	NS	NS	NS	NS	-
A:T to C:G	spontaneous	-	0.0001	0.027	NS	0.0005	0.0001	0.0001	NS
	DEB	0.0001	-	NS	0.03	NS	NS	NS	0.028
	DEB + GST (+ GSH)	0.027	NS	-	NS	NS	0.027	0.027	NS
	DEB-GSH	NS	0.03	NS	-	NS	0.007	0.007	NS
	EB	0.0005	NS	NS	NS	-	NS	NS	NS
	BD	0.0001	NS	0.027	0.007	NS	-	NS	0.012
	BD + microsomes	0.0001	NS	0.027	0.007	NS	NS	-	0.005
	BD + microsomes + GST (+ GSH)	NS	0.028	NS	NS	NS	0.012	0.005	-
A:T to G:C	spontaneous	-	0.0001	0.01	0.045	0.0001	0.0001	0.0001	0.0008
	DEB	0.0001	-	0.01	0.001	NS	NS	NS	0.01
	DEB + GST (+ GSH)	0.01	0.01	-	NS	0.01	0.013	0.012	NS
	DEB-GSH	0.045	0.001	NS	-	0.0013	0.0016	0.0014	NS
	EB	0.0001	NS	0.01	0.0013	-	NS	NS	0.01
	BD	0.0001	NS	0.013	0.0016	NS	-	NS	0.026
	BD + microsomes	0.0001	NS	0.012	0.0014	NS	NS	-	0.012
	BD + microsomes + GST (+ GSH)	0.0008	0.01	NS	NS	0.01	0.026	0.012	-

^aNS, not significant (P-value ≥ 0.05).