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. Author manuscript; available in PMC: 2013 Dec 3.
Published in final edited form as: Chem Res Toxicol. 2012 Jun 15;25(7):10.1021/tx3002109. doi: 10.1021/tx3002109

Table 4.

Quantitative Analysis of DNA Adducts in E. coli Cells Treated with DEB, DEB/GST/GSH, DEB-GSH Conjugate, or BD/Mouse Liver Microsomes/GST/GSH

adduct adducts per 105 bases
concentration 0.3 mM concentration 20%, in air (v/v)
DEB DEB + GST (+ GSH) DEB-GSH conjugate BD + mouse liver microsomes + GST (+ GSH)
(N = 2)a (N = 2) (N = 2) (N = 2)
N3A-(OH)2butyl-GSH NDb 0.93 ± 0.12 3.74 ± 0.59 0.10 ± 0.01
N6dA-(OH)2butyl-GSH ND 1.55 ± 0.42 6.01 ± 0.94 0.21 ± 0.04
N7G-(OH)2butyl-GSH ND 1.73 ± 0.26 6.84 ± 0.69 0.23 ± 0.14
N1dG-(OH)2butyl-GSH ND ND 0.45 ± 0.18 ND
N4dC-(OH)2butyl-GSH ND ND 0.98 ± 0.18 ND
N3dT-(OH)2butyl-GSH ND 0.62 ± 0.15 1.33 ± 0.30 ND
N7G-DEB 291 ± 46 305 ± 35 ND 12.2 ± 1.6
N3A-DEB 130 ± 6 135 ± 11 ND 6.9 ± 1.1
N6A-DEB 71 ± 4 68 ± 22 ND 4.2 ± 1.4
a

Means of duplicate experiments, ± range.

b

ND, not detected (limit of detection = 0.03 adducts/107 bases).