Table 1.
CODEML analysis of osmoregulatory genes (11 genes) and evidence of positive selection on the ACE, AGT, SLC14A2, REN, AQP2, and AVP genes in mammals and cetaceans
| Genes | Models | -lnL | 2ΔLnL | P valuea | ω values |
|---|---|---|---|---|---|
| ACE |
Site model |
||||
| (1033aa) |
Dataset I: All mammals (28 sequences) |
||||
| |
M8a |
20856.619 |
|
|
ω = 1 |
| |
M8 |
20853.691 |
5.856 |
0.016 |
ω = 2.077 |
| |
Dataset II: cetaceans (11 sequences) |
||||
| |
M8a |
5910.391 |
|
|
ω = 1 |
| |
M8 |
5905.151 |
10.480 |
0.001 |
ω = 4.855 |
| |
Branch site model |
||||
| |
Dataset I: All mammals (28 sequences) |
||||
| |
Branch n (terminal branch of Grampus griseus) |
||||
| |
Null |
21017.590 |
|
|
ω0 = 0.070, ω1 = 1, ω2 = 1 |
| |
Alternative |
21010.004 |
15.172 |
0.002 |
ω0 = 0.070, ω1 = 1, ω2 = 258.836 |
| AGT |
Site model |
||||
| (324aa) |
Dataset II: cetaceans (10 sequences) |
||||
| |
M8a |
2017.713 |
|
|
ω = 1 |
| |
M8 |
2014.489 |
6.448 |
0.011 |
ω = 4.134 |
| |
Branch site model |
||||
| |
Dataset I: All mammals (30 sequences) |
||||
| |
Branch n (terminal branch of G. griseus) |
||||
| |
Null |
10615.771 |
|
|
ω0 = 0.154, ω1 = 1, ω2 = 1 |
| |
Alternative |
10601.717 |
28.107 |
<0.001 |
ω0 = 0.154, ω1 = 1, ω2 = 952.146 |
| SLC14A2 |
Site model |
||||
| (861aa) |
Dataset I: All mammals (28 sequences) |
||||
| |
M8a |
15199.892 |
|
|
ω = 1 |
| |
M8 |
15197.706 |
4.372 |
0.037 |
ω = 1.529 |
| |
Dataset II: cetaceans (10 sequences) |
||||
| |
M8a |
4602.652 |
|
|
ω = 1 |
| |
M8 |
4597.947 |
9.410 |
0.002 |
ω = 2 |
| REN |
Site model |
||||
| (407aa) |
Dataset I: All mammals (25 sequences) |
||||
| |
M8a |
7542.944 |
|
|
ω = 1 |
| |
M8 |
7531.661 |
22.565 |
<0.001 |
ω = 16.110 |
| AQP2 |
Branch site model |
||||
| (202aa) |
Dataset I: All mammals (27 sequences) |
||||
| |
Branch c (ancestral toothed whales) |
||||
| |
Null |
3827.416 |
|
|
ω0 = 0.037, ω1 = 1, ω2 = 1 |
| Alternative | 3822.989 | 8.854 | 0.074 | ω0 = 0.038, ω1 = 1, ω2 = 999 | |