Table 1.
RT-PCR analysis for selected genes in chromosomal regions of deletion or amplification/duplication in primary RCC tumours and associated normal tissue
| Deletions | |||||
|---|---|---|---|---|---|
|
Chromosome |
Potential tumour specifically inactivated genes |
|
|||
| Tum Exp. Lost/Tum. Exp. Down-regulated/Tum. Exp. Normal or Up-regulated (Percentage of lost/down-regulated) |
|
||||
| Chromosome 1 |
CHD5 |
TNFRSF1B |
KIF17 |
UQCRH |
GPX7 |
| 0/1/14 (6.7%) |
0/0/15 (0.0%) |
0/0/15 (0.0%) |
0/0/15 (0.0%) |
0/0/15 (0.0%) |
|
| TTC22 |
PDE4DIP |
RGS7 |
TRIM58 |
|
|
| 1/1/13 (13.3%) |
0/0/15 (0.0%) |
3/4/8 (46.7%) |
0/2/13 (13.1%) |
|
|
| Chromosome 6 |
GCM2 |
HIST1H3G |
HIST1H4H |
|
|
| 8/0/2 (80.0%) |
0/0/15 (0.0%) |
0/0/15 (0.0%) |
|
|
|
| Chromosome 8p |
GATA4 |
NEFM |
|
|
|
| 1/0/8 (11.1%) |
1/2/12 (20.0%) |
|
|
|
|
| Chromosome 14q |
EFS |
PTGDR |
C14orf39 |
FLRT2 |
|
| 0/1/14 (6.7%) |
0/1/14 (6.7%) |
1/0/8 (11.1%) |
0/1/14 (6.7%) |
|
|
|
Amplifications | |||||
|
Chromosome |
Potential tumour specifically inactivated genes |
|
|||
| |
(Percentage of tumours in which gene expression is lost or down-regulated in brackets) |
|
|||
| Chromosome 1 |
TWIST1 |
HOXA11 |
AEBP1 |
KLF14 |
|
| |
1/0/13 (7.1%)1 |
0/2/13 (13.3%) |
3/0/12 (20.0%) |
0/2/13 (13.3%) |
|
| Chromosome 8p |
SOX17 |
CA3 |
ANGPT1 |
TMEM74 |
|
| 0/2/13 (13.3%) | 0/3/12 (20.0%) | 0/0/15 (0.0%) | 4/3/8 (46.7%) | ||
1Key= Tumour Expression Lost/Tumour Expression Down-regulated/Tumour Expression Normal or Up-regulated (Percentage of lost/down-regulated).
Genes in bold type (GCM2, RGS7 and NEFM) demonstrated complete loss or downregulation of expression in at least 20% of RCC tumours and were located in regions of deletion.