Table 1.
Antigenic characterisation of RHDV2 by ELISA using specific MAbs for RHDV and EBHSV reference strains.
| MAb | RHDV | RHDVa | EBHSV | RHDV2 | |
|---|---|---|---|---|---|
| i) |
2A10 |
100 |
0 |
0 |
0 |
| |
1H3 |
100 |
0 |
0 |
0 |
| |
1H8 |
100 |
0 |
0 |
0 |
| ii) |
3B12 |
0 |
100 |
0 |
0 |
| |
3D4 |
0 |
100 |
0 |
0 |
| |
3D6 |
0 |
100 |
0 |
0 |
| |
5D11 |
0 |
100 |
0 |
0 |
| |
2E1 |
0 |
100 |
0 |
0 |
| iii) |
2B4 |
100 |
100 |
0 |
0 |
| |
2G3 |
100 |
100 |
0 |
0 |
| |
1F10 |
100 |
100 |
0 |
15 |
| |
6H6 |
100 |
100 |
0 |
50 |
| |
3H2 |
100 |
100 |
0 |
100 |
| |
6D6 |
100 |
100 |
100 |
100 |
| |
2F9 |
100 |
100 |
100 |
100 |
| |
3H6 |
100 |
100 |
100 |
100 |
| iv) |
2B2 |
0 |
0 |
100 |
0 |
| |
1C5 |
0 |
0 |
100 |
0 |
| |
3D6 |
0 |
0 |
100 |
0 |
| |
4E3 |
0 |
0 |
100 |
0 |
| |
1F8 |
0 |
0 |
100 |
0 |
| |
5F5 |
0 |
0 |
100 |
0 |
| |
1G8 |
0 |
0 |
100 |
0 |
| |
1H1 |
0 |
0 |
100 |
0 |
| |
1H12 |
0 |
0 |
100 |
0 |
| 4H4 | 0 | 0 | 100 | 0 |
On the basis of their reactivity, the 26 monoclonal antibodies (MAb) were divided into 4 subsets. The reactivity of each MAb is given in percentage. Thus, 100 corresponds to a reactivity of 100% whereas 0 corresponds to an absence of reactivity.