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SDS-PAGE of expressed and purified Nk by ProBond™ Resin. A. Lane 1–5, culture supernatants; lane 6–9, cell lysates; lane 1,6, WB800/pAC7, lane 2–4, 7–9, WB800/pANk. Lane M, molecular standards indicated in kDa. B. Lane 1, purified Nk; Lane M, molecular standards indicated in kDa. The gel was stained by using Coomassie Briliant Blue R250. The band of lane 1 was cut off and used for LC-ESI-MS/MS analysis.
