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Purification steps of the nattokinase from B. subtilis WB800/pANk
| Step | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification factor | Yield (%) |
|---|---|---|---|---|---|
| Crude nattokinase |
24 |
4.8 |
5.0 |
1.0 |
100 |
| ProBond™ resin | 13 | 0.8 | 16.25 | 3.25 | 54.17 |
